Kev taw qhia rau iav Petri tais

Lub khob petri tais yog ib qho tseem ceeb ntawm cov khoom siv hauv chav kuaj siv dav hauv microbiology, cell biology, thiab lwm yam kev tshawb fawb txog lub neej. Muaj ob lub voj voog, tiaj tus -ib ntu loj hauv qab thiab lub hau me me, tshem tau lub hau uas haum rau nws- nws tau tsim los ntawm German bacteriologist Julius Richard Petri hauv 1887, yog li nws lub npe.

Cov khoom ntawm kev xaiv rau tsoos petri tais diav yogborosilicate iav. Hom iav no muaj ob peb yam tseem ceeb uas ua rau nws zoo tagnrho rau kev siv hauv chav kuaj. Ua ntej, nws muaj kev tiv thaiv thermal zoo heev, txhais tau tias nws tuaj yeem tiv taus qhov kub thiab txias hloov tsis tau tawg, uas yog qhov tseem ceeb rau cov txheej txheem xws li sterilization ntawm autoclaving (feem ntau ntawm 121℃thiab 15 psi siab). Qhov thib ob, borosilicate iav yog tshuaj inert; nws tsis hnov ​​​​mob nrog feem ntau cov kua qaub, cov hauv paus, lossis cov kuab tshuaj organic siv hauv kev sim, kom ntseeg tau tias qhov nruab nrab ntawm kev loj hlob thiab kev coj noj coj ua nyob tsis muaj kab mob los ntawm cov tshuaj tua kab mob.

Ib qho ntawm lub luag haujlwm tseem ceeb ntawm lub khob petri tais yog muab cov khoom tsis muaj menyuam, ib puag ncig rau kev cog qoob loo ntawm cov kab mob (xws li cov kab mob, cov kab mob, thiab cov poov xab) lossis eukaryotic hlwb. Ua ntej siv, lub tais yog sterilized kom tshem tawm tej yam tsis xav tau microbes. Tom qab ntawd, ib qho khoom noj khoom haus - nplua nuj nruab nrab, feem ntau yog agar, yog nchuav rau hauv qab thiab tso cai rau kom khov. Thaum nruab nrab txheej, cov qauv tuaj yeem raug inoculated rau nws qhov chaw siv cov tswv yim xws li streaking, kis, lossis pipetting. Lub hau yog muab tso rau saum toj kawg nkaus - tsis tau kaw tag nrho, kom tso cai rau kev sib pauv roj uas txhawb kev loj hlob ntawm microbial, tab sis snug txaus los tiv thaiv cov pa paug los ntawm kev nkag mus.

Muab piv rau lawv cov yas yas, iav petri tais diav muaj qhov zoo thiab qhov tsis zoo. Ntawm qhov ntxiv, lawv tuaj yeem rov siv tau- tom qab txhua qhov kev sim, lawv tuaj yeem raug ntxuav kom huv si, rov ua kom tsis muaj menyuam, thiab muab rov qab rau hauv kev pabcuam, uas txo cov nqi sijhawm ntev - thiab ib puag ncig pov tseg. Lawv kuj muab qhov pom kev pom zoo dua qub, ua kom yooj yim rau kev soj ntsuam thiab suav cov kab mob microbial nyob rau hauv lub tshuab tsom. Ntawm qhov downside, iav lauj kaub tais diav hnyav dua thiab yooj yim dua; lawv tuaj yeem tawg yog tias poob lossis ua yuam kev. Lawv kuj xav tau sijhawm thiab siv zog ntau dua rau kev ntxuav thiab ua kom tsis muaj menyuam piv rau cov tais diav yas pov tseg.

Ntxiv nrog rau kab lis kev cai microbial, iav petri tais diav muaj lwm yam siv ntau yam hauv chav kuaj. Lawv tuaj yeem ua cov thawv rau me me - cov tshuaj tiv thaiv kab mob, raws li lub nkoj tuav ib ntus rau cov qauv, lossis txawm tias yog lub platform rau kev sim cov noob germination hauv botany. Lawv lub tiaj tus, cov npoo tsis sib xws kuj ua rau lawv haum rau kev ua haujlwm ntawm microscopy, xws li tshuaj xyuas cov morphology ntawm cov kab lis kev cai.

Kev tuav thiab kev saib xyuas kom zoo yog qhov tseem ceeb los txuas ntxiv lub neej ntawm iav petri tais diav. Tom qab siv, lawv yuav tsum tau tsau rau hauv cov tshuaj tua kab mob tua cov kab mob uas tseem tshuav, tom qab ntawd maj mam txhuam kom tshem tawm cov seem nruab nrab, yaug nrog dej distilled, thiab qhuav ua ntej kom tsis muaj menyuam. Nws yog ib qho tseem ceeb uas yuav tau soj ntsuam lawv rau cov chips lossis cov kab nrib pleb ua ntej rov siv dua, vim tias cov tais diav puas tuaj yeem cuam tshuam tsis muaj menyuam thiab ua rau muaj kev phom sij.

Hauv cov ntsiab lus, iav petri phaj yog ib qho cuab yeej tseem ceeb hauv kev tshawb fawb tshawb fawb. Nws cov kav ntev, tshuaj lom neeg ruaj khov, thiab rov siv dua ua rau nws yog qhov tseem ceeb hauv cov chaw sim thoob ntiaj teb, ua lub luag haujlwm tseem ceeb hauv kev ua kom peb nkag siab txog cov kab mob thiab cov cellular lub neej.